Purification and Characterization of an endo-Polygalacturonase from the Gut of West Indies Sugarcane Rootstalk Borer Weevil ( Diaprepes abbreviatus L.) Larvae
Identifieur interne : 003324 ( Main/Exploration ); précédent : 003323; suivant : 003325Purification and Characterization of an endo-Polygalacturonase from the Gut of West Indies Sugarcane Rootstalk Borer Weevil ( Diaprepes abbreviatus L.) Larvae
Auteurs : Hamed Doostdar [États-Unis] ; T. Gregory Mccollum [États-Unis] ; Richard T. Mayer [États-Unis]Source :
- Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology [ 1096-4959 ] ; 1997.
English descriptors
- KwdEn :
Abstract
An endo-polygalacturonase (PG) (EC:3.2.1.15) with a pI of 9.4 and an Mr of 44,500 was purified to electrophoretic homogeneity from the gut of West Indies sugarcane rootstalk borer weevil (Diaprepes abbreviatus L.) larvae. Hydrolytic activity was maximal in 150 mM sodium acetate, pH 5.5, at 30°C. Kinetic determinations yielded an apparent Km of 3.68 mg polygalacturonic acid (PGA)/ml and a Vmax of 283 μmol galacturonic acid/min/mg protein for PGA. Enzymatic activity was inhibited by a polygalacturonase inhibitor protein from “Hamlin” orange flavedo. The purified protein does not appear to be glycosylated, and its N-terminal sequence showed no homology to any PG protein sequences in data banks.
Url:
DOI: 10.1016/S0305-0491(97)00285-X
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000B45
- to stream Istex, to step Curation: 000B45
- to stream Istex, to step Checkpoint: 000D01
- to stream Main, to step Merge: 003571
- to stream Main, to step Curation: 003324
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Purification and Characterization of an endo-Polygalacturonase from the Gut of West Indies Sugarcane Rootstalk Borer Weevil ( Diaprepes abbreviatus L.) Larvae</title><author><name sortKey="Doostdar, Hamed" sort="Doostdar, Hamed" uniqKey="Doostdar H" first="Hamed" last="Doostdar">Hamed Doostdar</name></author><author><name sortKey="Mccollum, T Gregory" sort="Mccollum, T Gregory" uniqKey="Mccollum T" first="T. Gregory" last="Mccollum">T. Gregory Mccollum</name></author><author><name sortKey="Mayer, Richard T" sort="Mayer, Richard T" uniqKey="Mayer R" first="Richard T" last="Mayer">Richard T. Mayer</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:6E070D9DA843F47BF288DD64A15BC16A7FD409DC</idno><date when="1997" year="1997">1997</date><idno type="doi">10.1016/S0305-0491(97)00285-X</idno><idno type="url">https://api.istex.fr/document/6E070D9DA843F47BF288DD64A15BC16A7FD409DC/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B45</idno><idno type="wicri:Area/Istex/Curation">000B45</idno><idno type="wicri:Area/Istex/Checkpoint">000D01</idno><idno type="wicri:doubleKey">1096-4959:1997:Doostdar H:purification:and:characterization</idno><idno type="wicri:Area/Main/Merge">003571</idno><idno type="wicri:Area/Main/Curation">003324</idno><idno type="wicri:Area/Main/Exploration">003324</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Purification and Characterization of an endo-Polygalacturonase from the Gut of West Indies Sugarcane Rootstalk Borer Weevil ( Diaprepes abbreviatus L.) Larvae</title><author><name sortKey="Doostdar, Hamed" sort="Doostdar, Hamed" uniqKey="Doostdar H" first="Hamed" last="Doostdar">Hamed Doostdar</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando</wicri:cityArea></affiliation></author><author><name sortKey="Mccollum, T Gregory" sort="Mccollum, T Gregory" uniqKey="Mccollum T" first="T. Gregory" last="Mccollum">T. Gregory Mccollum</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando</wicri:cityArea></affiliation></author><author><name sortKey="Mayer, Richard T" sort="Mayer, Richard T" uniqKey="Mayer R" first="Richard T" last="Mayer">Richard T. Mayer</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>U.S. Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, 2120 Camden Road, Orlando</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Comparative Biochemistry and Physiology, Part B: Biochemistry and Molecular Biology</title><title level="j" type="abbrev">CBBOLD</title><idno type="ISSN">1096-4959</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1997">1997</date><biblScope unit="volume">118</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="861">861</biblScope><biblScope unit="page" to="867">867</biblScope></imprint><idno type="ISSN">1096-4959</idno></series><idno type="istex">6E070D9DA843F47BF288DD64A15BC16A7FD409DC</idno><idno type="DOI">10.1016/S0305-0491(97)00285-X</idno><idno type="PII">S0305-0491(97)00285-X</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1096-4959</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus</term><term>N-terminal sequence</term><term>PGIP</term><term>herbivory</term><term>insect</term><term>plant defense</term><term>polygalacturonase inhibiting protein</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An endo-polygalacturonase (PG) (EC:3.2.1.15) with a pI of 9.4 and an Mr of 44,500 was purified to electrophoretic homogeneity from the gut of West Indies sugarcane rootstalk borer weevil (Diaprepes abbreviatus L.) larvae. Hydrolytic activity was maximal in 150 mM sodium acetate, pH 5.5, at 30°C. Kinetic determinations yielded an apparent Km of 3.68 mg polygalacturonic acid (PGA)/ml and a Vmax of 283 μmol galacturonic acid/min/mg protein for PGA. Enzymatic activity was inhibited by a polygalacturonase inhibitor protein from “Hamlin” orange flavedo. The purified protein does not appear to be glycosylated, and its N-terminal sequence showed no homology to any PG protein sequences in data banks.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Doostdar, Hamed" sort="Doostdar, Hamed" uniqKey="Doostdar H" first="Hamed" last="Doostdar">Hamed Doostdar</name></region><name sortKey="Mayer, Richard T" sort="Mayer, Richard T" uniqKey="Mayer R" first="Richard T" last="Mayer">Richard T. Mayer</name><name sortKey="Mccollum, T Gregory" sort="Mccollum, T Gregory" uniqKey="Mccollum T" first="T. Gregory" last="Mccollum">T. Gregory Mccollum</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003324 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003324 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:6E070D9DA843F47BF288DD64A15BC16A7FD409DC
|texte= Purification and Characterization of an endo-Polygalacturonase from the Gut of West Indies Sugarcane Rootstalk Borer Weevil ( Diaprepes abbreviatus L.) Larvae
}}
| This area was generated with Dilib version V0.6.25. |  |